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A whole medium alter was done daily, as well as cells were split yet again once a confluency of 80% was reached. All undifferentiated iPSC and human ESC colonies had been maintained in Critical eight medium (E8 medium; Existence Technologies) and incubated at 37°C in 5% CO2 humidified incubators. iPSCs and ESCs had been taken care of in T25 flasks
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